Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,282,409	A→G	P323P (CCA→CCG)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,282,409	0	A	G	100.0%	79.9 / NA	22	P323P (CCA→CCG)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/22);  total (0/22)

GCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTC  >  W3110S.gb/1282382‑1282437                            |                             gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1865191/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:876532/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:709083/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:639341/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:2357966/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:2179558/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:2175868/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:2141048/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:2109739/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:2033644/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1970522/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1015896/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1751443/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1653669/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1567196/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1538790/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:147957/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1424042/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1109866/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1077632/56‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1032076/56‑1 (MQ=255)                     aCAACCCGAGCCAGTATTTCACCGACTATGTGCGTc  <  1:1655729/36‑1 (MQ=255)                            |                             GCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTC  >  W3110S.gb/1282382‑1282437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑