Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,325,129	A→G	N66D (AAT→GAT)	yciV →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,325,129	0	A	G	100.0%	173.2 / NA	47	N66D (AAT→GAT)	yciV	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

GTTCGGGACTGGCGTTGAATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGT  >  W3110S.gb/1325112‑1325187                  |                                                           gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGaaa                                       >  1:1254203/1‑39 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAAtt                                     >  1:1313736/1‑41 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAAtt                                     >  1:1532005/1‑41 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCATGGTCTGGGAAAATCATGaa             >  1:1459004/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:301635/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:2276526/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:2179696/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:397469/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:2110748/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:573248/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:2376633/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:679140/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:1832519/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:1823031/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:350559/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:1687495/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:165851/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:1532649/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:907769/1‑65 (MQ=255) gTTCGGGACTGGCGTTGGATCTTATTAACGGCGTGGAAAt                                      >  1:2239927/1‑40 (MQ=37) gTTCGGGACTGGAGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGaa             >  1:130712/1‑65 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGATATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:386135/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:316819/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:67149/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:447272/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:46327/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:474856/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:600544/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:940780/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:760855/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:929922/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:1005244/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2475989/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2437519/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2363274/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2327090/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2252297/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2181541/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2134650/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:209621/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:2087923/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:1965813/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:1850456/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:1698225/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:1375841/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:1300718/63‑1 (MQ=255)              gTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGt  <  1:115868/63‑1 (MQ=255)                  |                                                           GTTCGGGACTGGCGTTGAATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAAAATCATGAAATTCATATTGT  >  W3110S.gb/1325112‑1325187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑