Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,346,872	C→T	V529I (GTT→ATT)	gmr ←	modulator of Rnase II stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,346,872	0	C	T	100.0%	89.6 / NA	26	V529I (GTT→ATT)	gmr	modulator of Rnase II stability
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/0);  total (26/0)

ATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCC  >  W3110S.gb/1346840‑1346903                                 |                                atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGttac  >  1:802844/1‑62 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1107501/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:868950/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:863529/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:797709/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:780449/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:665478/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:484484/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2401052/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2394893/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2386285/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2293629/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2196492/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2045574/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1976716/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1905900/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:184359/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1489879/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1450394/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1429954/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1282174/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1249019/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:11780/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:1033104/1‑64 (MQ=255) atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTca  >  1:872644/1‑63 (MQ=255) atTCTAAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTcc  >  1:2166176/1‑64 (MQ=255)                                 |                                ATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCC  >  W3110S.gb/1346840‑1346903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑