Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,475,513	A→G	pseudogene (8408/8622 nt) pseudogene (3110/3324 nt)	ydbA → ydbA →	ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,475,513	0	A	G	100.0%	120.6 / NA	33	pseudogene (8408/8622 nt) pseudogene (3110/3324 nt)	ydbA ydbA	ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/33);  total (0/33)

ACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAA  >  W3110S.gb/1475485‑1475567                             |                                                       aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:190587/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:432454/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:403086/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:287400/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:257578/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:494237/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:2369225/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:2301496/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:2217315/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:583343/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:692744/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1858407/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:185051/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1807500/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1515094/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1484507/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1351511/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1315159/65‑1 (MQ=255) aCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:1255893/65‑1 (MQ=255)             gggCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcaggcgc                    <  1:940322/53‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:2251913/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:705148/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:573326/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:709394/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:446379/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:2513625/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:2399321/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:1113397/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:2106575/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:1984685/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:1676464/65‑1 (MQ=255)                   aaCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:1149520/65‑1 (MQ=255)                     ccTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa  <  1:1454353/63‑1 (MQ=255)                             |                                                       ACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAA  >  W3110S.gb/1475485‑1475567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑