Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,496,286	A→G	L167P (CTT→CCT)	ydcI ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,496,286	0	A	G	100.0%	101.7 / NA	28	L167P (CTT→CCT)	ydcI	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/3);  total (25/3)

GGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCC  >  W3110S.gb/1496230‑1496316                                                         |                               ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:227293/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:947362/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:808676/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:738176/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:698749/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:495633/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:437715/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:40639/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:405613/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:2390478/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:2361863/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:1045725/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:2226087/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:2136293/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:2036265/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:1887275/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:1790964/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:1544463/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:1309320/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGtc                        >  1:1056163/1‑65 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGt                         >  1:185163/1‑64 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGt                         >  1:717516/1‑64 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGt                         >  1:1047687/1‑64 (MQ=255) ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGCtc                        >  1:1966722/1‑65 (MQ=255) ggTGATTAGGGAGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGt                         >  1:2430017/1‑64 (MQ=255)                       cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc  <  1:1827997/65‑1 (MQ=255)                       cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc  <  1:1408490/65‑1 (MQ=255)                        ttCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc  <  1:293877/64‑1 (MQ=255)                                                         |                               GGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCC  >  W3110S.gb/1496230‑1496316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑