Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,575,785	C→T	A378T (GCT→ACT)	pqqL ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,575,785	0	C	T	100.0%	130.0 / NA	39	A378T (GCT→ACT)	pqqL	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/26);  total (13/26)

GATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAT  >  W3110S.gb/1575725‑1575841                                                             |                                                         gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1131259/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:309825/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:584287/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:2261866/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:2218757/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:714591/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1971155/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1943733/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:177754/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:170430/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1704179/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1666714/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1424636/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1375203/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1355335/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1352384/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:943688/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:1234983/65‑1 (MQ=255) gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTGCTTCAGTAGaa                                                      <  1:389124/65‑1 (MQ=255)  aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:510805/64‑1 (MQ=255)  aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:382503/64‑1 (MQ=255)                            ggCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                      <  1:2124020/38‑1 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:716508/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:800453/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:494355/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:952307/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:331186/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:2421205/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:2360380/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:2202807/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:1552740/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:1406090/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag     >  1:1256562/1‑65 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgca      >  1:864065/1‑64 (MQ=255)                                                  gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTAgcag     >  1:1160919/1‑65 (MQ=255)                                                     ttcttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAt  <  1:501757/65‑1 (MQ=255)                                                     ttcttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAt  <  1:2352656/65‑1 (MQ=255)                                                     ttcttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAt  <  1:706064/65‑1 (MQ=255)                                                     ttcttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAt  <  1:871517/65‑1 (MQ=255)                                                             |                                                         GATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAT  >  W3110S.gb/1575725‑1575841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑