| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,609,975 | C→T | L254L (CTG→CTA) | yneE ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,609,975 | 0 | C | T | 100.0% | 11.8 / NA | 5 | L254L (CTG→CTA) | yneE | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) | |||||||||||
AATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAA > W3110S.gb/1609932‑1609996 | aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:1956868/1‑65 (MQ=255)aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:1973486/1‑65 (MQ=255)aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:2224614/1‑65 (MQ=255)aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:763388/1‑65 (MQ=255)aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:76707/1‑65 (MQ=255) | AATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAA > W3110S.gb/1609932‑1609996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |