Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,642,131	A→G	Y57H (TAT→CAT)	essQ ←	predicted S lysis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,642,131	0	A	G	100.0%	97.6 / NA	27	Y57H (TAT→CAT)	essQ	predicted S lysis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/0);  total (27/0)

CGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTA  >  W3110S.gb/1642096‑1642171                                    |                                         cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:1341813/1‑64 (MQ=255) cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:969591/1‑64 (MQ=255) cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:548917/1‑64 (MQ=255) cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:460177/1‑64 (MQ=255) cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:302415/1‑64 (MQ=255) cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:2371785/1‑64 (MQ=255) cGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCa              >  1:2318022/1‑64 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCt   >  1:1029646/1‑59 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:1585252/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:809929/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:805099/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:771885/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:755632/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:621834/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:597267/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:57865/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:1473115/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:2184992/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:379996/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:31638/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:1720648/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:2411591/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:2401323/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:2376751/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:1930358/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:2020426/1‑60 (MQ=255)                 tcttctTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTa  >  1:2218792/1‑60 (MQ=255)                                    |                                         CGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTA  >  W3110S.gb/1642096‑1642171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑