Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,794,138	A→T	V103D (GTC→GAC)	nlpC ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,794,138	0	A	T	97.1%	103.2 / ‑3.7	35	V103D (GTC→GAC)	nlpC	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (0/34);  total (0/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

TTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTG  >  W3110S.gb/1794078‑1794179                                                             |                                          ttgttgGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCa                                        <  1:187799/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1744392/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:856642/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:803078/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:762800/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:717286/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:599736/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:424772/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:390854/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:386652/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:2517122/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:2349288/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:2019863/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1717940/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1708445/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1534191/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1003196/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1110523/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1140940/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:114308/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1332068/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1334929/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1380319/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1395826/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1403844/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1444880/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:14841/64‑1 (MQ=255)                                       tCCGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1504067/64‑1 (MQ=255)                                        ccGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:2439489/63‑1 (MQ=255)                                        ccGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:2145341/63‑1 (MQ=255)                                        ccGGACCCCGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1954069/63‑1 (MQ=255)                                              cccGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1680365/57‑1 (MQ=255)                                               ccGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:2447169/56‑1 (MQ=255)                                                cGTTTTGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:1106710/55‑1 (MQ=255)                                                    ttGAAAAAGTCCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTg  <  1:966933/51‑1 (MQ=255)                                                             |                                          TTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTG  >  W3110S.gb/1794078‑1794179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑