| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,799,263 | G→A | S132S (AGC→AGT) | pheT ← | phenylalanine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,799,263 | 0 | G | A | 100.0% | 14.3 / NA | 7 | S132S (AGC→AGT) | pheT | phenylalanine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/0); total (7/0) | |||||||||||
GATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATG > W3110S.gb/1799218‑1799281 | gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAAt > 1:1973607/1‑63 (MQ=255)gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAAt > 1:2218376/1‑63 (MQ=255)gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:1035735/1‑64 (MQ=255)gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:1140598/1‑64 (MQ=255)gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:162231/1‑64 (MQ=255)gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:2453375/1‑64 (MQ=255)gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:2487835/1‑64 (MQ=255) | GATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATG > W3110S.gb/1799218‑1799281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |