Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	330,966	T→G	intergenic (+246/‑629)	betT → / → yahA	choline transporter of high affinity/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	330,966	0	T	G	100.0%	173.8 / NA	47	intergenic (+246/‑629)	betT/yahA	choline transporter of high affinity/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (47/0);  total (47/0)

GTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTA  >  W3110S.gb/330921‑331022                                              |                                                         gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAAc                                         >  1:1326922/1‑63 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAAc                                         >  1:1987799/1‑63 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAAc                                         >  1:1483124/1‑63 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:725719/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1012654/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2303517/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2342890/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2389368/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2421261/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:347948/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:400158/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:435500/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:481389/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:64091/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2218579/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:786004/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:876412/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:890329/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:906071/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:918102/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:921096/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:950208/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:969450/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1827931/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1398432/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1521054/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1392581/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:15665/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1231004/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2184866/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1750179/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1782018/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1170303/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1920821/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1076592/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:1964427/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2075517/1‑64 (MQ=255) gTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:105168/1‑64 (MQ=255) gTAAATATTGCCGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACt                                        >  1:2343713/1‑64 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:163550/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:952659/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:78976/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:1572461/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:350595/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:339589/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:2028703/1‑65 (MQ=255)                                      aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa  >  1:2160497/1‑65 (MQ=255)                                              |                                                         GTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTA  >  W3110S.gb/330921‑331022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑