Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,807,728	C→T	A24T (GCT→ACT)	ydiY ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,807,728	0	C	T	100.0%	94.5 / NA	29	A24T (GCT→ACT)	ydiY	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/19);  total (10/19)

TTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAA  >  W3110S.gb/1807677‑1807788                                                    |                                                             ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTg                                                   >  1:1056862/1‑63 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:358715/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:362944/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:2434012/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:383852/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:500607/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:609783/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:1558660/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:1450363/1‑64 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa                                                  >  1:705212/1‑64 (MQ=255)                                                 cAGTTGCATTCAGTTACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:71002/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:622852/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:60811/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:424407/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:412785/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:750815/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:875239/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:2513260/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:201913/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1992393/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1990945/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1950795/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1895300/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1881384/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1874683/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1371234/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:121071/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1074807/64‑1 (MQ=255)                                                 cAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa  <  1:1071676/64‑1 (MQ=255)                                                    |                                                             TTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAA  >  W3110S.gb/1807677‑1807788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑