Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,810,661	A→G	F96S (TTT→TCT)	yniB ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,810,661	0	A	G	100.0%	100.6 / NA	27	F96S (TTT→TCT)	yniB	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/27);  total (0/27)

CCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAA  >  W3110S.gb/1810627‑1810691                                   |                               ccGGGCACCGGAATCCTGCAACGCCAGTCCGATAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:2322367/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:2062395/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:883669/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:746429/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:701410/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:66272/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:636048/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:622016/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:405134/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:286605/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:2502086/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:2217381/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:209890/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:2074496/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1065065/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:2031672/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:200334/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1844623/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1737475/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1669284/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1660903/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1611992/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1593963/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1465301/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1218714/65‑1 (MQ=255) ccGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1131263/65‑1 (MQ=255)  cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa  <  1:1393191/64‑1 (MQ=255)                                   |                               CCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAA  >  W3110S.gb/1810627‑1810691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑