Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,853,161	G→A	P196P (CCG→CCA)	ansA →	cytoplasmic L‑asparaginase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,853,161	0	G	A	100.0%	157.5 / NA	50	P196P (CCG→CCA)	ansA	cytoplasmic L‑asparaginase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

CCTCCGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTA  >  W3110S.gb/1853105‑1853215                                                         |                                                       cctccGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCACACGGTGa                                                >  1:766858/1‑65 (MQ=255) cctccGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCACACGGTGa                                                >  1:720580/1‑65 (MQ=255) cctccGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCACACGCTGa                                                >  1:483589/1‑65 (MQ=255)                                 tcttttGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:5194/4‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGTGAATTAATCGTTCATCCAATCACCCcac                >  1:776406/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCaa                         >  1:833482/1‑56 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCAccc                   >  1:1991763/1‑62 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:2388027/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:989566/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:75053/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:70391/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:516609/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:2498283/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:2386517/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:2225461/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:1040055/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:1491933/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:1572906/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:1662382/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac                >  1:1746864/1‑65 (MQ=255)                                 tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCca                 >  1:264882/1‑64 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1702998/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:2091804/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:976740/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:2144428/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1586348/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1625239/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:2251333/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1916300/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1931582/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1035664/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:228140/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:2349561/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:239318/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:2460476/65‑1 (MQ=255)                                          tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1810769/65‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGTGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:74472/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACATCCAATTg       <  1:434567/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:5205/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:613173/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:216831/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:154982/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1851107/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:1384727/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:904084/64‑1 (MQ=255)                                           aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg       <  1:2519952/64‑1 (MQ=255)                                                aCCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTa  >  1:2415758/1‑64 (MQ=255)                                                aCCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTa  >  1:2207742/1‑64 (MQ=255)                                                aCCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTa  >  1:2513011/1‑64 (MQ=255)                                                aCCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTa  >  1:895137/1‑64 (MQ=255)                                                         |                                                       CCTCCGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTA  >  W3110S.gb/1853105‑1853215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑