Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,865,587	T→C	L8L (CTT→CTC)	yeaD →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,865,587	0	T	C	100.0%	31.8 / NA	10	L8L (CTT→CTC)	yeaD	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/10);  total (0/10)

TAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGG  >  W3110S.gb/1865569‑1865633                   |                                               tAAGAAAATTTTTGCCCTCCCGGTCATCGAACATATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:1797781/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:1751818/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:1948906/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:2132785/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:2226897/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:2287311/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:2299116/65‑1 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:618298/65‑1 (MQ=255)  aaGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:1379191/64‑1 (MQ=255)  aaGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTgg  <  1:2096919/64‑1 (MQ=255)                   |                                               TAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGG  >  W3110S.gb/1865569‑1865633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑