Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,990,800	A→G	intergenic (+118/‑53)	yecR → / → ftn	hypothetical protein/cytoplasmic ferritin iron storage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,990,800	0	A	G	100.0%	225.9 / NA	60	intergenic (+118/‑53)	yecR/ftn	hypothetical protein/cytoplasmic ferritin iron storage protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/60);  total (0/60)

TCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCA  >  W3110S.gb/1990742‑1990847                                                           |                                                tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:366558/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1827801/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1879154/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:2238358/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:2258317/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:2263998/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:2373876/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:242026/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:2441108/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:297631/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1135321/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:52464/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:722247/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:757995/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:76783/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:790748/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:837436/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:840575/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:882306/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:912089/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1533982/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1328425/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1266222/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1261222/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1257693/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1210314/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1563461/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1603233/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1693845/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:108020/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1652135/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1682971/64‑1 (MQ=255) tCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:1205471/64‑1 (MQ=255)  cccATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:249030/63‑1 (MQ=255)                              tataTTTTACCTTTTGCAAATAATAAAATGACaaa                                            <  1:565530/35‑1 (MQ=255)                                         ttttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGa     <  1:1480987/63‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTTGAGCa  <  1:1437112/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:950030/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1293738/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1278658/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:761291/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:987747/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1705586/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:903443/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:856311/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:478339/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:380560/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1402686/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:342174/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1463289/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:241130/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:148863/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:223509/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:2025203/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1933129/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1901675/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1796072/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:1708942/65‑1 (MQ=255)                                          tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGACCa  <  1:325805/65‑1 (MQ=255)                                           ttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:57622/64‑1 (MQ=255)                                                    ataaaatgaCAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCa  <  1:288217/55‑1 (MQ=255)                                                           |                                                TCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCA  >  W3110S.gb/1990742‑1990847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑