Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,000,742	C→T	M292I (ATG→ATA)	yedO ←	D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,000,742	0	C	T	100.0%	268.3 / NA	79	M292I (ATG→ATA)	yedO	D‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/76);  total (3/76)

GAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAA  >  W3110S.gb/2000687‑2000770                                                        |                             gAATCGGCCCTTCATCTTTTAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:224357/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:595185/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:249796/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:2148015/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:216598/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:363121/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1841570/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1794500/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:2180291/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1759403/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1693488/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1680199/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:550804/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1557768/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:627123/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:640145/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:783165/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1254862/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:724332/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:706044/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1285286/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:681790/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:65213/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:641979/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1453865/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1539658/65‑1 (MQ=255) gAATCGGCCCTTCATCTTTGAAGCGTTTCCGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1461294/65‑1 (MQ=255)     cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:450028/65‑1 (MQ=255)     cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:446997/65‑1 (MQ=255)     cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:1678555/65‑1 (MQ=255)     cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:380971/65‑1 (MQ=255)     cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:2024841/65‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:242939/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2341272/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:248383/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2401174/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:992587/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2512417/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2518427/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:307838/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:351004/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:445865/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:56819/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:811849/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:965743/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:102062/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1652818/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:102864/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1102265/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1163297/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1192634/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1279552/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1283200/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1349372/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1351824/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1445978/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1514829/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1552886/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:157945/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1612522/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2329245/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1757722/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1761006/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1822684/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1883543/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1932015/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1692/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1940212/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:203424/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2121313/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2144242/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2166470/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2217181/64‑1 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCa   >  1:401205/1‑63 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCa   >  1:628020/1‑63 (MQ=255)                     aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCa   >  1:633975/1‑63 (MQ=255)                     aaGCGTTTCCGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2299542/64‑1 (MQ=255)                     aaGCGTTCCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:954033/64‑1 (MQ=255)                                                aGCCCCGCTATCGCTTTTCCGGTATTCACAGGATCaa  <  1:333692/37‑1 (MQ=38)                                                        |                             GAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAA  >  W3110S.gb/2000687‑2000770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑