Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	367,904	G→T	G24C (GGC→TGC)	mhpA →	3‑(3‑hydroxyphenyl)propionate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	367,904	0	G	T	87.5%	35.9 / 1.0	16	G24C (GGC→TGC)	mhpA	3‑(3‑hydroxyphenyl)propionate hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (14/0);  total (14/2)
Fisher's exact test for biased strand distribution p-value = 8.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGT  >  W3110S.gb/367852‑367956                                                     |                                                     tGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGc                                          <  1:1325296/65‑1 (MQ=255) tGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGACGGCCCGGTTGGGc                                          <  1:1712711/65‑1 (MQ=255)                                         atggCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:323062/4‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACg   >  1:1111749/1‑64 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACg   >  1:242316/1‑64 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:1150837/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:1200304/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:1242062/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:1794800/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:1805553/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:2011398/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:2094705/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:2097511/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:2144502/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:2216146/1‑65 (MQ=255)                                         aTCGCTGGTGCCTGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGt  >  1:2534130/1‑65 (MQ=255)                                                     |                                                     TGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGT  >  W3110S.gb/367852‑367956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑