Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,039,900	A→G	I130I (ATT→ATC)	yedV ←	predicted sensory kinase in two‑component regulatory system with YedW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,039,900	0	A	G	100.0%	325.2 / NA	85	I130I (ATT→ATC)	yedV	predicted sensory kinase in two‑component regulatory system with YedW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/55);  total (30/55)

AGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTC  >  W3110S.gb/2039864‑2039940                                     |                                         aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1602034/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1980035/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1961329/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1919258/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:2061677/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1825852/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:2155915/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1775714/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:2275544/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:2407788/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1656259/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:164188/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1629615/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:2509154/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:994389/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1149872/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:150143/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:932944/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:559653/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1282724/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:821130/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1504053/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1314102/65‑1 (MQ=255) aGTAACCGTTAATGATGGCGAAACTCCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:626246/65‑1 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGt                                    >  1:432481/1‑38 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTa                  >  1:1621014/1‑56 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:213209/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:2364906/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:20881/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:927282/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:2030227/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:92688/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:2153298/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:695683/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:2432299/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:566291/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:110326/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:289384/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:549132/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1906483/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1592327/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1861007/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1845611/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1164509/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1272919/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1666759/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:1288821/1‑65 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat          >  1:367950/1‑64 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat          >  1:2536342/1‑64 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat          >  1:2475997/1‑64 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat          >  1:89501/1‑64 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat          >  1:950305/1‑64 (MQ=255)      ccGTTAATGATGGCGAAACTTCATCAAAATTGATTCGTAAAGCATCTATCTCTGTATCATtaata         >  1:234272/1‑65 (MQ=255)      ccGATAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATc                >  1:454891/1‑58 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTTTCTCTGTATCATTAATAATGCTTc  <  1:985648/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:747735/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:744465/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:140039/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1422018/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1427932/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1434814/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1556549/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:121194/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:444493/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1577807/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1886178/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2527540/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2073959/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2145333/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1819780/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:177651/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2165812/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2199572/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2201352/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2265965/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1765656/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2338655/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1731355/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1594468/64‑1 (MQ=255)              gatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:429893/64‑1 (MQ=255)               atgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:1102895/63‑1 (MQ=255)                tgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAt              <  1:1685278/50‑1 (MQ=255)                   cGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2339855/59‑1 (MQ=255)                       aCTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2074514/55‑1 (MQ=255)                        cTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTc  <  1:2443585/54‑1 (MQ=255)                                     |                                         AGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTC  >  W3110S.gb/2039864‑2039940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑