Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,096,084	G→A	E160E (GAG→GAA)	hisB →	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,096,084	0	G	A	100.0%	170.9 / NA	49	E160E (GAG→GAA)	hisB	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/44);  total (5/44)

CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT  >  W3110S.gb/2096045‑2096119                                        |                                    cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc                             >  1:1051159/1‑48 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc                             >  1:112195/1‑48 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc                             >  1:1182495/1‑48 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc                             >  1:1522888/1‑48 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc                             >  1:358224/1‑48 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2212605/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:952472/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2218298/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2267715/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2328722/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2347994/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2491046/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2519597/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2530293/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:320267/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:546119/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:606233/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:662127/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:729570/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:733405/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:73342/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:795866/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:798094/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:800926/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:912460/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1042981/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1646473/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1084864/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1232391/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:124534/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1298754/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1349040/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1494675/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1521851/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1645460/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2132676/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1648403/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1690434/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1735106/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1815868/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1921915/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:200006/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2037208/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2075051/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2093380/65‑1 (MQ=255)           gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2111381/65‑1 (MQ=255)             aaCCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:666951/63‑1 (MQ=255)                 cTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:675167/59‑1 (MQ=255)                     aCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:120317/55‑1 (MQ=255)                                        |                                    CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT  >  W3110S.gb/2096045‑2096119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑