Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,257,455 C→T G9R (GGA→AGA)  yeiJ ← predicted nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,257,4550CT100.0% 482.5 / NA 138G9R (GGA→AGA) yeiJpredicted nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (65/73);  total (65/73)

Only 100 of 138 total aligned reads displayed.

TTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGC  >  W3110S.gb/2257406‑2257514
                                                 |                                                           
ttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCa                                               >  1:2220764/1‑64 (MQ=255)
ttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCAt                                              >  1:1011315/1‑65 (MQ=255)
ttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCAt                                              >  1:264790/1‑65 (MQ=255)
ttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCAt                                              >  1:1555222/1‑65 (MQ=255)
ttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCAt                                              >  1:571029/1‑65 (MQ=255)
ttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCAt                                              >  1:11878/1‑65 (MQ=255)
    tGTTTACTTACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:512121/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACAGTTCTCATGa                                            <  1:2092422/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2087319/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2154078/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:207598/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:207463/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:225114/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:226630/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2069959/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2383730/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2411216/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2475068/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2477239/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2508526/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2184180/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1967267/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:625979/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:913516/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:912540/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:836460/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:815324/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:784821/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:764908/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:760451/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:646371/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2522575/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:617231/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:548443/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:45567/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:436577/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:402076/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:351659/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:315263/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1768451/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1319/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1372277/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1389943/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1424799/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1463338/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1249001/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1519415/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1209943/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1543387/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1653022/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1684973/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1842606/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1105605/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1955943/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1040203/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1892482/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1874304/63‑1 (MQ=255)
    tGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1176006/63‑1 (MQ=255)
               aGTTAATACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1050255/52‑1 (MQ=37)
                             cGTCAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:1878043/38‑1 (MQ=255)
                                cAGCAATACCACCATTCTCAGAACACTTCTCATGa                                            <  1:2473292/35‑1 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGAtt                              >  1:875004/1‑47 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCatat             >  1:33299/1‑64 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1052057/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:658898/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1054179/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:663755/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1274977/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:875704/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:903408/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:653064/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:708139/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:607418/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1281786/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:987288/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:2024625/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1946508/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1003781/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1490817/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:2537263/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:219286/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1776690/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:2193413/1‑65 (MQ=255)
                                  gCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:1839009/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCa                >  1:274944/1‑51 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATAtt            >  1:502610/1‑55 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:710880/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:1835153/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:1115683/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:1714599/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:496377/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:2173315/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:868719/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:1901517/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:1909106/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:581167/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:2032936/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:986683/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:1990089/1‑65 (MQ=255)
                                            caTTCTCAGAACACTTCGCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc  >  1:132079/1‑65 (MQ=255)
                                                 |                                                           
TTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGC  >  W3110S.gb/2257406‑2257514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑