Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,271,945	A→G	R261R (CGA→CGG)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,271,945	0	A	G	100.0%	43.5 / NA	13	R261R (CGA→CGG)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/0);  total (13/0)

AATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGT  >  W3110S.gb/2271935‑2271998           |                                                      aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTgg   >  1:2264376/1‑63 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1013680/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1031061/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1131143/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1139454/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1546542/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:180786/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1891316/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:2221412/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:2294626/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:382882/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:625322/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTACCGAAGGGCTGGt  >  1:784488/1‑64 (MQ=255)           |                                                      AATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGT  >  W3110S.gb/2271935‑2271998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑