Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,281,208	A→G	L525L (CTA→CTG)	yejF →	fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,281,208	0	A	G	100.0%	182.9 / NA	49	L525L (CTA→CTG)	yejF	fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/32);  total (17/32)

AAGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTACTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATT  >  W3110S.gb/2281153‑2281263                                                        |                                                        aaGGAGCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                <  1:1082292/65‑1 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGtt                                                 >  1:1101293/1‑64 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                <  1:2102874/65‑1 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                >  1:2323749/1‑65 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                <  1:493843/65‑1 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                <  1:1456832/65‑1 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                >  1:785085/1‑65 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                >  1:845966/1‑65 (MQ=255) aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACCCGTCAGCTGCTGGCGTTg                                                <  1:38684/65‑1 (MQ=255)  aGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg                                                <  1:1604460/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:705132/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:946168/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:2046821/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:1948045/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:2482598/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:409419/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:518954/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:53289/64‑1 (MQ=255)                           acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg                       <  1:1382421/64‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:396645/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:588294/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:822838/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:654836/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:2325892/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:682774/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:2054589/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1020173/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:2006446/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1137696/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1201531/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:126478/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1293513/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1408459/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1607554/65‑1 (MQ=255)                                       gAGTATACGCGTCAGCTGCTGGCATTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1458694/65‑1 (MQ=255)                                        aGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt          <  1:1796438/64‑1 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:1615757/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:982011/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:120144/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:125423/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:1483281/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:2084534/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:474670/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:1619695/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:1758695/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:362223/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:2411020/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt  >  1:2266025/1‑64 (MQ=255)                                                cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAt   >  1:1855176/1‑63 (MQ=255)                                                        |                                                        AAGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTACTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATT  >  W3110S.gb/2281153‑2281263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑