Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,426,403	G→A	G113R (GGG→AGG)	yfcG →	predicted glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,426,403	0	G	A	100.0%	92.4 / NA	29	G113R (GGG→AGG)	yfcG	predicted glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/0);  total (29/0)

TGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGC  >  W3110S.gb/2426364‑2426428                                        |                          tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTTATCACGc  >  1:302403/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCCCg   >  1:2450970/1‑64 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACg   >  1:1302846/1‑64 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACg   >  1:2063118/1‑64 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:211142/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:956162/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:900021/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:659331/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:488966/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:340732/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2476443/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2460742/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2392824/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2260952/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:224059/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2210445/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2181090/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1026853/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2077141/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:2017099/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1936359/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1770158/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1719501/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1711065/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1537684/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1296152/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:122847/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:117310/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc  >  1:1168820/1‑65 (MQ=255)                                        |                          TGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGC  >  W3110S.gb/2426364‑2426428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑