Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,452,377	C→T	P181P (CCG→CCA)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,452,377	0	C	T	100.0%	156.1 / NA	46	P181P (CCG→CCA)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

AACTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTC  >  W3110S.gb/2452345‑2452415                                 |                                       aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCg         <  1:946357/64‑1 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCg         <  1:1287603/64‑1 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCg         <  1:1471596/64‑1 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCg         <  1:896437/64‑1 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCg         <  1:1695401/64‑1 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACgg                          >  1:1387075/1‑43 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:514116/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:1802081/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:450986/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:332972/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:1992963/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:302567/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:2073078/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:278269/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:2175353/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:2250320/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:2394263/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:1772802/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:1764804/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:703835/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:770437/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:171887/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:1654786/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:1268133/1‑64 (MQ=255)     cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:965817/1‑64 (MQ=255)     cGTCTAACGAGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCa     >  1:2121108/1‑64 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:616414/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:2033555/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:418926/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:347325/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:2414549/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:2226823/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:2167522/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1047142/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:198239/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1957771/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1954487/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1917681/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1861219/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1825992/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:174297/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1735834/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1692095/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1150990/64‑1 (MQ=255)        cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:1134911/64‑1 (MQ=255)         tAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc  <  1:811992/63‑1 (MQ=255)                                 |                                       AACTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTC  >  W3110S.gb/2452345‑2452415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑