Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,469,991	T→C	S155G (AGC→GGC)	vacJ ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,469,991	0	T	C	100.0%	265.9 / NA	71	S155G (AGC→GGC)	vacJ	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/69);  total (2/69)

CAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACG  >  W3110S.gb/2469930‑2470031                                                              |                                         cAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCAc                                        >  1:18628/1‑64 (MQ=255) cAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCAc                                        >  1:2330970/1‑64 (MQ=255)            cGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGT‑‑AACGGTAAc                          <  1:1996280/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:222204/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1930741/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1940880/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1951450/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1992289/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2086053/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2120999/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2166114/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2212046/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1182855/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2252912/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2337180/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:351579/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:357670/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:383072/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:728789/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:934079/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:941285/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:983361/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1279089/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1406440/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1728098/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1408498/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:123286/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1591966/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:159906/65‑1 (MQ=255)              ggTAAAAACCATCCGCCATATCACCACCATCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:1649210/65‑1 (MQ=255)               gTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGGGAAGCCACCGTAGAACGGTAAc                          <  1:643120/64‑1 (MQ=255)                tAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:2168735/63‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGCACGGTAACTGAACGTAAGGCCCATAAccc     <  1:2277062/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:167102/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:1437386/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:296316/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:1039300/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:2534082/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:1629745/64‑1 (MQ=255)                                    accaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:2520848/64‑1 (MQ=255)                                     ccaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAAc                          <  1:448030/42‑1 (MQ=255)                                     ccaccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAccc     <  1:422154/63‑1 (MQ=255)                                      caccGTCATCACGCATCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:226535/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:58770/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:991673/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1276460/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1252785/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:426977/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:522318/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:562917/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:58953/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:236557/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:682208/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1209464/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1214684/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:294952/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2538871/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2502629/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2469083/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1737417/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2358853/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1285321/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1321057/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2294160/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2244069/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2230690/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1518956/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1641160/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1706103/65‑1 (MQ=255)                                      caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1825346/65‑1 (MQ=255)                                       accGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1746120/64‑1 (MQ=255)                                                              |                                         CAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACG  >  W3110S.gb/2469930‑2470031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑