BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,512,230	A→G	W371R (TGG→CGG)	ypdG ←	predicted enzyme IIC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,512,230	0	A	G	100.0%	378.4 / NA	101	W371R (TGG→CGG)	ypdG	predicted enzyme IIC component of PTS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (77/24); total (77/24)

Only 100 of 101 total aligned reads displayed.

CTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAC  >  W3110S.gb/2512170‑2512290

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCTGCCATGCCCGGATa                                                          >  1:2292179/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:2491977/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:1719107/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:1997525/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:2017612/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:684360/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:688743/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:2429196/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:928533/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:1118218/1‑65 (MQ=255)

cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATa                                                          >  1:1099993/1‑65 (MQ=255)

                 cccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                         <  1:1789744/65‑1 (MQ=255)

                 cccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                         <  1:8751/65‑1 (MQ=255)

                 cccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                         <  1:1597307/65‑1 (MQ=255)

                  ccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                         <  1:1279628/64‑1 (MQ=255)

                  ccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                         <  1:1427479/64‑1 (MQ=255)

                             aCGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                         <  1:2079463/53‑1 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCTGACTCCGGGAACCATTGCACTGCGccc                            >  1:701979/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:1872200/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:631235/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:1875526/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:629893/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:2062906/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:2079006/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:221517/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:251235/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:2542447/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:2521858/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:2396377/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:2409718/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:1055722/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:1715709/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:117588/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:1687248/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:633497/1‑65 (MQ=255)

                              cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                            >  1:1404778/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGCCTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1360675/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCTCCCAGCCAGACAgcgg               >  1:1435036/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2131571/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1767458/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2170077/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2229681/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:502495/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:406922/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:227976/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1315879/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:259684/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1236506/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:832317/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2466524/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1720695/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1706091/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1864529/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:209541/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1429676/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2067749/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2062263/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:2034050/1‑65 (MQ=255)

                                           gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcgg               >  1:1657650/1‑65 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:647882/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:849114/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1227344/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1952847/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:783607/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1961530/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:604228/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:2166734/65‑1 (MQ=255)

                                                    ccATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:589745/65‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCATACAGCGGCGGTTGAGc      <  1:2150146/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1073222/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:620299/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1088813/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1180891/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:2252625/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:2108843/64‑1 (MQ=255)

                                                     cATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGc      <  1:1685911/64‑1 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGACCCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:760834/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTgcg                               >  1:733246/1‑35 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGa   >  1:891897/1‑63 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGa   >  1:1389056/1‑63 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1861705/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:914468/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1111568/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1181084/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1270184/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1289579/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1389152/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1602492/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:245952/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:1932289/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2124684/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:59717/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2148410/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2251228/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:274707/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2308537/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2344473/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2538026/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc  >  1:2351964/1‑64 (MQ=255)

                                                         cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGACAGACAGCGGCGGTTGAGCCGAc  >  1:349069/1‑64 (MQ=255)

CTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAC  >  W3110S.gb/2512170‑2512290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`