Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,559,548	T→C	I293T (ATT→ACT)	hemF →	coproporphyrinogen III oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,559,548	0	T	C	100.0%	97.1 / NA	27	I293T (ATT→ACT)	hemF	coproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/27);  total (0/27)

GGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGG  >  W3110S.gb/2559491‑2559564                                                          |                 gggAATATTATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2383895/65‑1 (MQ=255) gggAATATGATTATCAGCCATAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2072981/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:878171/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:1005314/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:668262/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:48622/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:450639/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:383483/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2390332/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2380188/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2367970/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2319640/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2249538/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:2078958/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:1602614/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:1472542/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:1347287/65‑1 (MQ=255) gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:1317096/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:819667/56‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:2010930/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:2456640/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:450405/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:619885/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:627407/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:844031/65‑1 (MQ=255)          aTTATCAGCCAAAAGATGGCAGACCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg  <  1:703363/65‑1 (MQ=255)                           ggCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc           <  1:1347595/39‑1 (MQ=255)                                                          |                 GGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGG  >  W3110S.gb/2559491‑2559564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑