Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,560,022	G→A	P203S (CCT→TCT)	yfeG ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,560,022	0	G	A	100.0%	59.4 / NA	20	P203S (CCT→TCT)	yfeG	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/0);  total (20/0)

AATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTC  >  W3110S.gb/2559992‑2560056                               |                                   aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt   >  1:576226/1‑64 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:2052553/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:98896/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:899187/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:87470/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:492684/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:2307029/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:2222299/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:2181791/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:198943/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1828360/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1766186/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1646585/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1618461/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1335102/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1274217/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1113520/1‑65 (MQ=255) aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:100664/1‑65 (MQ=255) aaTTATCCCCAAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt   >  1:835702/1‑64 (MQ=255) aaTTAACCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc  >  1:1004137/1‑65 (MQ=255)                               |                                   AATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTC  >  W3110S.gb/2559992‑2560056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑