| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,620,788 | A→G | E312E (GAA→GAG) | purM → | phosphoribosylaminoimidazole synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,620,788 | 0 | A | G | 100.0% | 20.8 / NA | 7 | E312E (GAA→GAG) | purM | phosphoribosylaminoimidazole synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (3/4); total (3/4) | |||||||||||
ATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCG > W3110S.gb/2620732‑2620833 | aTGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAGGTGGACaa < 1:511305/65‑1 (MQ=255)aTGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAGGTGGACaa < 1:744482/65‑1 (MQ=255)aTGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAGGTGGACaa < 1:82183/65‑1 (MQ=255)aTGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTACGGAGGTGGACaa < 1:1977304/65‑1 (MQ=255) ttGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAAcgcg > 1:622265/1‑65 (MQ=255) ttGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAAcgcg > 1:922095/1‑65 (MQ=255) ttGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAAcgcg > 1:953140/1‑65 (MQ=255) | ATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCG > W3110S.gb/2620732‑2620833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |