| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,732,756 | T→A | D25V (GAC→GTC) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,732,756 | 0 | T | A | 100.0% | 14.2 / NA | 6 | D25V (GAC→GTC) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (6/0); total (6/0) | |||||||||||
TCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCG > W3110S.gb/2732724‑2732785 | tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:1964225/1‑62 (MQ=255)tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:2228566/1‑62 (MQ=255)tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:2228910/1‑62 (MQ=255)tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:388895/1‑62 (MQ=255)tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:527095/1‑62 (MQ=255)tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:812888/1‑62 (MQ=255) | TCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCG > W3110S.gb/2732724‑2732785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |