Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,841,701	C→T	L134L (CTG→CTA)	hycH ←	protein required for maturation of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,841,701	0	C	T	100.0%	220.1 / NA	63	L134L (CTG→CTA)	hycH	protein required for maturation of hydrogenase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/28);  total (35/28)

CCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGA  >  W3110S.gb/2841659‑2841740                                           |                                        cccATCATGCTATTGCGAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1210665/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAg                                       >  1:1196535/1‑45 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:529101/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1898441/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2041134/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:211455/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2147893/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2160361/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2211398/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2216041/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2437409/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:424872/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:496916/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1065610/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:538657/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:591490/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:64700/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:650856/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:693864/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:733993/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:759978/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:85268/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1162055/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1205580/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:135845/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1162119/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:137931/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1495523/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1546066/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1120097/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1628155/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1259208/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1226695/1‑65 (MQ=255) cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTAACGTAGTCTCCTCACCATCAAATAGa                   >  1:2465327/1‑65 (MQ=255)          cTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGtttt          >  1:510742/1‑65 (MQ=255)                  gacacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:332844/64‑1 (MQ=255)                  aaCACAGAGTAAAACTTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:788970/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:578873/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:620464/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:505804/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1186293/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:674964/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:718754/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:722981/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1017119/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:905800/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:981186/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:56643/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:542043/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1238031/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1864908/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1359148/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:362619/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:300640/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:2498332/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:2428594/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:139495/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1404116/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1612160/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:2083984/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1992409/65‑1 (MQ=255)                  aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1680879/65‑1 (MQ=255)                   acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1959218/64‑1 (MQ=255)                                           |                                        CCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGA  >  W3110S.gb/2841659‑2841740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑