| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,792,109 | Δ1 bp | coding (317/1320 nt) | uhpC → | membrane protein regulates uhpT expression |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,792,107 | 0 | G | . | 95.1% | 148.0 / 2.9 | 41 | coding (315/1320 nt) | uhpC | membrane protein regulates uhpT expression |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (11/28); total (12/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
GTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCT > W3110S.gb/3792054‑3792163 | gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:935621/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:3048642/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:2883515/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:2590977/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:376951/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:485514/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:209927/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:2079032/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:1879600/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:917385/1‑71 (MQ=255)gTCAGCGATCGCTCAAATGCCCGTTATTATATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:711840/1‑71 (MQ=255) aaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:604253/47‑1 (MQ=255) aaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:623118/47‑1 (MQ=255) aaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:633457/47‑1 (MQ=255) aaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:2810122/47‑1 (MQ=255) aaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:1467843/47‑1 (MQ=255) aaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:3168950/47‑1 (MQ=255) aTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:1884012/46‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:3630699/43‑1 (MQ=39) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:988098/43‑1 (MQ=39) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:1621104/43‑1 (MQ=39) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATc < 1:1625787/43‑1 (MQ=39) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:413015/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:1131114/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:1254737/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:672186/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:1815382/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:3103177/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:3819512/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:273809/71‑1 (MQ=255) cccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:3253490/71‑1 (MQ=255) ccGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:4063095/71‑1 (MQ=255) ccGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGc < 1:2102070/70‑1 (MQ=255) tAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGt > 1:4000759/1‑70 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTGGCTATGGGCGTTTGCCGTGct < 1:151795/70‑1 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct < 1:2460160/70‑1 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct < 1:3727598/70‑1 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct < 1:3628700/70‑1 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct < 1:731510/70‑1 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct < 1:35678/70‑1 (MQ=255) ttATCGCCAC‑GGCATTATCAACATTCTGTTTGGCt < 1:1694026/35‑1 (MQ=37) | GTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCT > W3110S.gb/3792054‑3792163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |