| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,989,743 | (G)7→6 | intergenic (‑617/+12) | yhiS ← / ← arsC | hypothetical protein/arsenate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,989,737 | 0 | G | . | 81.8% | 47.2 / 14.0 | 22 | intergenic (‑611/+18) | yhiS/arsC | hypothetical protein/arsenate reductase |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base . (5/13); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTGGGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGCCATCTTC > W3110S.gb/3989673‑3989802 | ggTCGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTgggggga > 1:2745512/1‑70 (MQ=255) tCGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTggggggat < 1:1860328/70‑3 (MQ=255) cGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTGGGGGGGata > 1:4077969/1‑71 (MQ=255) cGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTGGGGGGGata > 1:1389939/1‑71 (MQ=255) cGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTGGGGGGGata > 1:3058315/1‑71 (MQ=255) gcgcCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTGGGGGGGATATCGCCGCtt < 1:1788310/70‑1 (MQ=255) gcgcCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTa < 1:2482940/71‑1 (MQ=255) aTCCGACATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTa > 1:890559/1‑71 (MQ=255) aTCCGACATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTa > 1:1437080/1‑71 (MQ=255) aCATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGc < 1:251991/71‑1 (MQ=255) aCATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGc < 1:3218420/71‑1 (MQ=255) aCATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGc < 1:3005152/71‑1 (MQ=255) aCATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGc < 1:2797247/71‑1 (MQ=255) aCATAAATAAAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGc < 1:1121186/71‑1 (MQ=255) ataaataaAGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCtt < 1:2946369/71‑1 (MQ=255) ataaaGCGCACTTTTCTAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGc < 1:4077553/65‑1 (MQ=255) tAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTtctc < 1:584504/67‑1 (MQ=255) tAACAACCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTtctc < 1:1285429/67‑1 (MQ=255) acaacCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTtctc < 1:2451526/65‑1 (MQ=255) acaacCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGc > 1:1317548/1‑67 (MQ=255) acaacCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGCCa < 1:1767683/69‑1 (MQ=255) acaacCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGCCa < 1:1539704/69‑1 (MQ=255) acCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGCCATCTTc > 1:2394646/1‑71 (MQ=255) acCTGT‑GGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGCCATCTTc > 1:46749/1‑71 (MQ=255) | GGTCGGATAAGACGCGCCAGCGTCGCATCCGACATAAATAAAGCGCACTTTTCTAACAACCTGTGGGGGGGATATCGCCGCTTTATTTCAGGCGCTTACCCGCTTCATCAACCACTTTCTCGCCATCTTC > W3110S.gb/3989673‑3989802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |