Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,371,276 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,371,2730A.100.0% 120.0 / NA 30intergenic (‑6/+300)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/26);  total (4/26)
*W3110S.gb4,371,2740A.100.0% 120.0 / NA 30intergenic (‑7/+299)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/26);  total (4/26)

TACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGG  >  W3110S.gb/4371207‑4371321
                                                                  ||                                               
tACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaa                                                >  1:377802/1‑69 (MQ=255)
        agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                    <  1:3640259/71‑1 (MQ=255)
        agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                    <  1:2655785/71‑1 (MQ=255)
         gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                   <  1:970301/71‑1 (MQ=255)
         gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                   <  1:2325137/71‑1 (MQ=255)
         gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                   <  1:1753841/71‑1 (MQ=255)
         gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                   <  1:1437414/71‑1 (MQ=255)
          cagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                   <  1:2192021/70‑1 (MQ=255)
           agcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATaa                                  <  1:739956/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGTGATAACCGCCATGATAATGgag                 <  1:3990461/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:1910692/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:918611/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:20087/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:1470258/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:2527589/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:2866425/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:3909769/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:3164995/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:1336671/70‑1 (MQ=255)
                            aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:3423405/70‑1 (MQ=255)
                             aCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                 <  1:1010958/69‑1 (MQ=255)
                                    aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa             >  1:3321241/1‑66 (MQ=255)
                                    aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa             >  1:2241813/1‑66 (MQ=255)
                                    aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa             >  1:2219757/1‑66 (MQ=255)
                                    aGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa             >  1:187941/1‑66 (MQ=255)
                                      tCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                    <  1:440158/41‑1 (MQ=38)
                                      tCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                    <  1:649195/41‑1 (MQ=38)
                                          gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg  <  1:1019621/71‑1 (MQ=255)
                                          gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg  <  1:3089182/71‑1 (MQ=255)
                                          gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg  <  1:901979/71‑1 (MQ=255)
                                          gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg  <  1:1994335/71‑1 (MQ=255)
                                                                  ||                                               
TACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGG  >  W3110S.gb/4371207‑4371321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑