Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,381,932	Δ1 bp	coding (331/759 nt)	yfbB ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,381,930	0	C	.	100.0%	93.6 / NA	24	coding (333/759 nt)	yfbB	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (4/20);  total (4/20)

GCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCA  >  W3110S.gb/2381863‑2381974                                                                    |                                             gCTGCACCCTTTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:825019/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:1010640/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:765180/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:1766680/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:2569549/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:1804612/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:1889765/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:2172563/71‑3 (MQ=255) gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:254251/71‑3 (MQ=255)         cATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccg                                            <  1:2993850/62‑2 (MQ=255)             ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATa                               <  1:417034/70‑1 (MQ=255)             ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATa                               <  1:4045767/70‑1 (MQ=255)                 aTCGGAACGCTGACGTTCCGCACGTTGTTCATCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:304787/59‑1 (MQ=255)                 aTCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:520563/59‑1 (MQ=255)                   cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCca                         >  1:2726218/1‑70 (MQ=255)                                ttCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc                                           <  1:3368234/40‑3 (MQ=255)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:943913/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:3705588/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:78697/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:3248031/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:310151/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:2608130/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:2400004/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc                                      <  1:2391169/41‑1 (MQ=39)                                   cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGcc         <  1:1749432/70‑1 (MQ=255)                                     cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGa                                    >  1:1551465/1‑41 (MQ=39)                                     cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGa                                    >  1:1484163/1‑41 (MQ=39)                                     cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGa                                    >  1:844467/1‑41 (MQ=39)                                     cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCt      <  1:2627550/71‑1 (MQ=255)                                     cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCt      <  1:1778831/71‑1 (MQ=255)                                         ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa  <  1:3175350/71‑1 (MQ=255)                                         ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa  <  1:3908481/71‑1 (MQ=255)                                         ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa  <  1:462441/71‑1 (MQ=255)                                         ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa  <  1:942433/71‑1 (MQ=255)                                         ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa  <  1:1042240/71‑1 (MQ=255)                                                                    |                                             GCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCA  >  W3110S.gb/2381863‑2381974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑