Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,824,537 +G pseudogene (670/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,5331.G100.0% 43.3 / NA 15pseudogene (666/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/8);  total (7/8)

GGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGAGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTTAAGGCGACT  >  W3110S.gb/3824470‑3824597
                                                                |                                                                
ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAgggggaa                                                           >  1:76762/1‑68 (MQ=255)
     cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                             <  1:1648494/64‑2 (MQ=255)
     cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                             <  1:1191242/64‑2 (MQ=255)
     cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                             <  1:3851030/64‑2 (MQ=255)
     cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                             <  1:1803480/64‑2 (MQ=255)
     cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                             <  1:3185453/64‑2 (MQ=255)
     cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                             <  1:3007664/64‑2 (MQ=255)
       gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAgggggaa                                                           >  1:3768949/1‑61 (MQ=255)
       gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAgggggaa                                                           >  1:4122760/1‑61 (MQ=255)
       gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAgggggaa                                                           >  1:2424052/1‑61 (MQ=255)
       gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAgggggaa                                                           >  1:1792237/1‑61 (MQ=255)
          gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                                     >  1:2572014/1‑68 (MQ=255)
          gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                                     >  1:969814/1‑68 (MQ=255)
          gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                                     >  1:4070215/1‑68 (MQ=255)
          gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                                     >  1:1884782/1‑68 (MQ=255)
          gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                                     >  1:3297078/1‑68 (MQ=255)
          gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                                     >  1:3419799/1‑68 (MQ=255)
                                     cTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                                         <  1:714754/37‑1 (MQ=21)
                                     cTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                                         <  1:997401/37‑1 (MQ=21)
                                     cTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                                         <  1:2986256/37‑1 (MQ=21)
                                     cTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                                         <  1:2034645/37‑1 (MQ=21)
                                     cTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                                         <  1:1686837/37‑1 (MQ=21)
                                           accTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt                  >  1:3095601/3‑70 (MQ=255)
                                                gttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAtt              <  1:2252404/69‑1 (MQ=255)
                                                          gCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTTAAGGCGACt  <  1:2882666/71‑1 (MQ=255)
                                                          gCCCGAGGGGGAATCGAATCCATTGTAGCGACACAGAAGGCGGCGCTGGCAAACTGATTTTTAAGGCGACt  <  1:3552676/71‑1 (MQ=255)
                                                                |                                                                
GGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGAGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTTAAGGCGACT  >  W3110S.gb/3824470‑3824597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: