| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,155,147 | +A | coding (782/870 nt) | prkB ← | predicted phosphoribulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,155,146 | 1 | . | A | 85.7% | 124.9 / 19.7 | 49 | coding (783/870 nt) | prkB | predicted phosphoribulokinase |
| Reads supporting (aligned to +/- strand): ref base . (6/1); new base A (23/19); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGAAATCG > W3110S.gb/4155091‑4155209 | gCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGa < 1:2409174/70‑1 (MQ=255) attaattCCATTGCCAGACCCATTTTGGCGCCCGGTACCAACTAACGTATTGATGTGGGa < 1:1413494/60‑1 (MQ=255) attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGa < 1:705734/60‑1 (MQ=255) attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGa < 1:50144/60‑1 (MQ=255) attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGcc > 1:3575909/1‑71 (MQ=255) attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGcc > 1:3202360/1‑71 (MQ=255) attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGcc > 1:2339539/1‑71 (MQ=255) taattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGaaa > 1:1119027/1‑60 (MQ=255) taattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGaaa > 1:3619951/1‑60 (MQ=255) taattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGaaa > 1:2050843/1‑60 (MQ=255) aattCCATTGCCAGACCCATTTTGCCTCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:582501/70‑1 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:500502/70‑1 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:4007617/70‑1 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:1334307/70‑1 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:2283685/70‑1 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:3403899/70‑1 (MQ=255) aattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAATGATGTGGGAAATGAATGAGCCt < 1:1685739/70‑1 (MQ=255) attCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCt < 1:2029384/69‑1 (MQ=255) ttCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGc < 1:3507575/71‑1 (MQ=255) ttCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGc < 1:1941337/71‑1 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAAc > 1:1389844/1‑36 (MQ=37) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAAGGTATTGATGTGGGAAATGAATGAGCCTTGCAACAt > 1:564716/1‑71 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAAc > 1:3351476/1‑69 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACa > 1:419458/1‑70 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACAt > 1:1051918/1‑71 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACAt > 1:1743599/1‑71 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACAt > 1:1139396/1‑71 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACAt > 1:451458/1‑71 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACAt > 1:326547/1‑71 (MQ=255) cAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGcc < 1:560091/71‑1 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGATTGAGCCTTGCAACATCGCcag > 1:3972043/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:339028/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:1472342/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:2883878/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:1366085/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:1824025/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:4016784/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:1291055/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:3583410/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:1657884/1‑70 (MQ=255) aCCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCcag > 1:1684801/1‑70 (MQ=255) ttttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc < 1:668319/49‑1 (MQ=39) ttGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGATTGAGCCTTGCat > 1:3896121/1‑52 (MQ=255) ttGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCaa > 1:2286318/1‑53 (MQ=255) ttGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCaa > 1:306052/1‑53 (MQ=255) ccgccCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGa < 1:3995763/71‑1 (MQ=255) ccgccCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGa < 1:3311553/71‑1 (MQ=255) ccgccCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGa < 1:3565396/71‑1 (MQ=255) ccGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGAAATCg < 1:3613623/71‑1 (MQ=255) | GCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGGGAAATCG > W3110S.gb/4155091‑4155209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |