Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,428,265	+G	coding (738/855 nt)	ulaE →	L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,428,262	1	.	G	100.0%	175.1 / NA	48	C245W (TGC→TGG)	ulaE	L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (8/40);  total (8/40)

CCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGA  >  W3110S.gb/4428197‑4428303                                                                   |                                          ccGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑ggggc                                      >  1:3053074/1‑69 (MQ=255)              gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga                          <  1:2550784/71‑1 (MQ=255)              gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga                          <  1:2097794/71‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:970822/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:2931226/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:3184775/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:3527896/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:3604063/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:3632749/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:376339/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:3990818/70‑1 (MQ=255)                 tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga                        <  1:807164/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAGACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:3640687/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:352664/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:3415512/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:664739/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:1292267/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:2920433/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:2739016/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:1374653/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:1587520/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:152183/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:2341999/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:23825/70‑1 (MQ=255)                   gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:2670250/70‑1 (MQ=255)                      gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa                  <  1:213597/71‑1 (MQ=255)                      gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa                  <  1:222044/71‑1 (MQ=255)                      gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa                  <  1:3051710/71‑1 (MQ=255)                      gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGa                  <  1:2234664/71‑1 (MQ=255)                        tttCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGc                >  1:3846693/1‑71 (MQ=255)                             aaCGTTGTTTCGAATCGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga                          <  1:93637/56‑1 (MQ=255)                                  tgttTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtg                      <  1:2520543/55‑1 (MQ=255)                                    tttCGAAACGCTGAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:2849487/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:3908627/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:3929452/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:4088060/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:474511/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:3471366/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:3374734/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:56013/52‑1 (MQ=255)                                    tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAt                       <  1:3059805/52‑1 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:750507/1‑52 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:1028045/1‑52 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:328097/1‑52 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:2725008/1‑52 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:3132254/1‑52 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:3075606/1‑52 (MQ=255)                                      tCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt                     >  1:2898369/1‑52 (MQ=255)                                       cGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGa  <  1:2909021/70‑1 (MQ=255)                                                                   |                                          CCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGA  >  W3110S.gb/4428197‑4428303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑