| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,595,970 | (C)8→9 | coding (3831/3978 nt) | ydeK ← | predicted lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,595,962 | 1 | . | C | 75.0% | 26.3 / 4.2 | 12 | coding (3839/3978 nt) | ydeK | predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (4/5); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
ACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAG > W3110S.gb/1595895‑1596020 | acCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccc > 1:191870/1‑71 (MQ=255)acCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccc > 1:628946/1‑71 (MQ=255)acCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccc > 1:2812498/1‑71 (MQ=255)acCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccc > 1:2903769/1‑71 (MQ=255)acCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccc > 1:343821/1‑71 (MQ=255) ttATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccccccccc < 1:355519/70‑2 (MQ=255) tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcg < 1:1920788/68‑1 (MQ=255) tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcg < 1:2871427/68‑1 (MQ=255) tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcg < 1:3680605/68‑1 (MQ=255) tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑ccc > 1:3907654/1‑49 (MQ=255) gACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGa > 1:1076009/1‑71 (MQ=255) gACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGa > 1:2214830/1‑71 (MQ=255) gACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGa > 1:502219/1‑71 (MQ=255) aCTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcg < 1:765294/63‑1 (MQ=255) tatTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGa < 1:2981732/63‑1 (MQ=255) agagTCGGAACCGAAAGCAATTTCCCCCCCCCCCGATATCcgtc > 1:1919148/1‑44 (MQ=255) agagTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCgttg > 1:3559048/1‑71 (MQ=255) agagTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCgttg > 1:2546221/1‑71 (MQ=255) gAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAg > 1:1304769/1‑70 (MQ=255) | ACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAG > W3110S.gb/1595895‑1596020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |