| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,035,876 | (T)8→9 | intergenic (‑255/‑312) | yedR ← / → yedS | predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,035,868 | 1 | . | T | 95.0% | 51.5 / ‑2.7 | 20 | intergenic (‑247/‑320) | yedR/yedS | predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (5/14); total (5/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
TGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATT > W3110S.gb/2035800‑2035930 | tgtCATGATGCTTCCGTTATTAGCCTTTTATCCTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tt > 1:651591/1‑71 (MQ=255) atgCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt > 1:1014775/1‑70 (MQ=255) atgCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt > 1:2520838/1‑70 (MQ=255) gCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑ttttttttta > 1:606110/1‑68 (MQ=255) tCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGcc > 1:463024/1‑71 (MQ=255) tCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGcc > 1:3334020/1‑71 (MQ=255) cGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCtt < 1:166727/71‑1 (MQ=255) cGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCtt < 1:2129012/71‑1 (MQ=255) cGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCtt < 1:2761422/71‑1 (MQ=255) cGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCtt < 1:300031/71‑1 (MQ=255) aGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTaa < 1:1235954/70‑1 (MQ=255) aGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTaa < 1:3880495/70‑1 (MQ=255) gTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:2425381/71‑1 (MQ=255) gTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATca < 1:1802931/68‑1 (MQ=255) tataTTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAg > 1:2901009/1‑71 (MQ=255) tataTTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAg > 1:3269929/1‑71 (MQ=255) tataTTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAg > 1:3516474/1‑71 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:255201/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:2351046/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:2345328/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:793290/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:875656/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:9076/71‑1 (MQ=255) atgCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:3658269/70‑1 (MQ=255) | TGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATT > W3110S.gb/2035800‑2035930 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |