| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,169,967 | (T)7→8 | intergenic (‑10/+159) | yegZ ← / ← yegR | ECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,169,960 | 1 | . | T | 87.0% | 53.8 / 5.0 | 23 | intergenic (‑3/+166) | yegZ/yegR | ECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base T (3/17); total (3/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTC > W3110S.gb/2169892‑2170024 | cGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt < 1:2913509/71‑1 (MQ=255)cGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt < 1:2761784/71‑1 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:3252477/70‑3 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:3935762/70‑3 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:3635649/70‑3 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:2286137/70‑3 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:3279851/70‑3 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:2574222/70‑3 (MQ=255) ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:3392163/70‑3 (MQ=255) atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGc < 1:2465469/71‑1 (MQ=255) atacCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGc < 1:348346/71‑1 (MQ=255) cGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATctct < 1:3222734/71‑1 (MQ=255) cGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGACCTATctct < 1:2349417/71‑1 (MQ=255) gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt < 1:4072324/40‑1 (MQ=255) cAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tttttttta < 1:2422052/42‑3 (MQ=255) ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:2278314/71‑1 (MQ=255) gCTGATGGAGAACTCTGCTACATGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:2103438/70‑1 (MQ=255) gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTAtctc < 1:1089822/56‑1 (MQ=39) gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:2301973/70‑1 (MQ=255) gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:2297405/70‑1 (MQ=255) tGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:2371890/68‑1 (MQ=255) gaACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACca > 1:1130059/1‑70 (MQ=255) gaACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACca > 1:2932385/1‑70 (MQ=255) gaACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAAACGTCTTCCATTGACca > 1:1223750/1‑70 (MQ=255) aaCTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACcaca < 1:3157136/71‑1 (MQ=255) aaCTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACcaca < 1:3284782/71‑1 (MQ=255) aaCTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACcaca < 1:1903071/71‑1 (MQ=255) aaCTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACcaca < 1:1897885/71‑1 (MQ=255) aaCTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACcaca < 1:4012355/71‑1 (MQ=255) ctctGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACcaca < 1:2558095/69‑1 (MQ=255) tGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGa < 1:2727927/69‑1 (MQ=255) tGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGa < 1:2047789/69‑1 (MQ=255) tGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGa < 1:3609492/69‑1 (MQ=255) ggCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAAtcctc < 1:3815806/71‑1 (MQ=255) | CGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTC > W3110S.gb/2169892‑2170024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |