Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,298,699	(T)5→6	coding (16/480 nt)	ccmE ←	periplasmic heme chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,298,694	1	.	T	100.0%	48.1 / NA	19	N7K (AAC→AAA)	ccmE	periplasmic heme chaperone
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (0/19);  total (0/19)

GCAGCGCATATAGCACCAGACCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGG‑TTTTTACGGCGAATATTCATGCAGCCT  >  W3110S.gb/2298624‑2298721                                                                        |                            gcagcgcaTATAGCACCAGACCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCgg                              <  1:2194372/71‑1 (MQ=255)                    aCCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGAATATTc           <  1:1474804/71‑1 (MQ=255)                    aCCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGAATATTc           <  1:862229/71‑1 (MQ=255)                    aCCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGAATATTc           <  1:7601/71‑1 (MQ=255)                       gATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:214669/63‑1 (MQ=255)                       gATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:3601726/63‑1 (MQ=255)                       gATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:2680957/63‑1 (MQ=255)                       gATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:2065288/63‑1 (MQ=255)                       gATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:2764383/63‑1 (MQ=255)                       gATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:3003564/63‑1 (MQ=255)                       gATAGGCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:2511093/63‑1 (MQ=255)                        aTAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCgg                              <  1:309093/48‑1 (MQ=255)                                           ccAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:2756558/43‑1 (MQ=38)                                           ccAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:807726/43‑1 (MQ=38)                                           ccAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:1530662/43‑1 (MQ=38)                                           ccAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:3526133/43‑1 (MQ=38)                                           ccAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:261680/43‑1 (MQ=38)                                           ccAACACGGCACAGGCAATCCACAAGCGGTTTTTTACGGCGaa                <  1:1845302/43‑1 (MQ=38)                                                            aTCCACAAGCGGTTTTTTACGGCGAATATTCATGCAGcct  <  1:2670536/40‑1 (MQ=38)                                                            aTCCACAAGCGGTTTTTTACGGCGAATATTCATGCAGcct  <  1:2665738/40‑1 (MQ=38)                                                            aTCCACAAGCGGTTTTTTACGGCGAATATTCATGCAGcct  <  1:1084819/40‑1 (MQ=38)                                                                        |                            GCAGCGCATATAGCACCAGACCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGG‑TTTTTACGGCGAATATTCATGCAGCCT  >  W3110S.gb/2298624‑2298721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑