Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb47,8280AG73.1% 88.6 / 34.5 53V20V (GTA→GTGkefCpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (5/9);  new base G (24/14);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCG  >  W3110S.gb/47769‑47897
                                                           |                                                                     
atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCAt                                                                  >  1:467425/1‑65 (MQ=255)
atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCAt                                                                  >  1:1717223/1‑65 (MQ=255)
atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATAGTGCCCa                                                                   >  1:901196/1‑64 (MQ=255)
   gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACg                                                         >  1:3391436/1‑71 (MQ=255)
     tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTc                                                       >  1:53057/1‑71 (MQ=255)
     tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTc                                                       >  1:3254947/1‑71 (MQ=255)
     tAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTc                                                       >  1:2703735/1‑71 (MQ=255)
      aGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCt                                                      >  1:1454975/1‑71 (MQ=255)
       gCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCtt                                                     >  1:618189/1‑71 (MQ=255)
       gCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCtt                                                     >  1:4089342/1‑71 (MQ=255)
       gCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCtt                                                     >  1:3513715/1‑71 (MQ=255)
       gCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCtt                                                     >  1:3225192/1‑71 (MQ=255)
       gCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCtt                                                     >  1:3223312/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTAGGTCTTGGTCTg                                               >  1:1271281/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCt                                                >  1:737780/1‑70 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:3419672/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:1021147/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:1375442/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:4013574/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:37786/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:3561627/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:3316479/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:3273654/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:1967480/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                               >  1:2204191/1‑71 (MQ=255)
             cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCATTGCGGTACGTCTTGGTCTgg                                              >  1:4135812/1‑71 (MQ=255)
               cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg                                             <  1:1986604/71‑1 (MQ=255)
               cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg                                             <  1:2143473/71‑1 (MQ=255)
               cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg                                             <  1:2983011/71‑1 (MQ=255)
               cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg                                             <  1:876450/71‑1 (MQ=255)
               cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg                                             <  1:2429231/71‑1 (MQ=255)
                   ttCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGAt                                           >  1:1409392/1‑69 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACTTCTTGGTCTGGGATCGGTa                                      <  1:1626969/71‑1 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                      <  1:1649403/71‑1 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                      <  1:302296/71‑1 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                      <  1:2655964/71‑1 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                      <  1:1789528/71‑1 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                      <  1:4107590/71‑1 (MQ=255)
                      aGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                      <  1:324052/71‑1 (MQ=255)
                         cgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCTGTACGTCTTGGTCTGGGATCGGTACtt                                   >  1:2663004/1‑71 (MQ=255)
                         cgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTTGTCTGGGATCGGTACtt                                   >  1:3522823/1‑71 (MQ=255)
                         cgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACtt                                   >  1:2154254/1‑71 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:1308565/70‑1 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:609717/70‑1 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:525446/70‑1 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:3151992/70‑1 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:3714153/70‑1 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:3378418/70‑1 (MQ=255)
                              aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                               <  1:2984166/70‑1 (MQ=255)
                               tttATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAc                             <  1:1615183/71‑1 (MQ=255)
                               tttATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTAc                             <  1:3707679/71‑1 (MQ=255)
                                                          tACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCg  <  1:3513543/71‑1 (MQ=255)
                                                          tACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCg  <  1:3371219/71‑1 (MQ=255)
                                                           |                                                                     
ATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCG  >  W3110S.gb/47769‑47897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: