Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,032,4910CT50.0% ‑5.3 / 30.2 22intergenic (‑357/‑70)serT/hyaAtRNA‑Ser/hydrogenase 1, small subunit
Reads supporting (aligned to +/- strand):  ref base C (11/0);  new base T (6/5);  total (17/5)
Fisher's exact test for biased strand distribution p-value = 3.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAG  >  W3110S.gb/1032442‑1032543
                                                 |                                                    
tGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt                                         <  1:2928354/63‑1 (MQ=255)
tGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt                                         <  1:3735165/63‑1 (MQ=255)
  aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATATGGACGTCGCTTGCCGACCAt                                >  1:366319/1‑70 (MQ=255)
  aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt                                >  1:1740431/1‑70 (MQ=255)
  aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt                                >  1:4038596/1‑70 (MQ=255)
  aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt                                >  1:3985053/1‑70 (MQ=255)
  aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt                                >  1:217984/1‑70 (MQ=255)
  aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt                                >  1:270171/1‑70 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:313322/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:606869/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:4082860/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:3982703/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:3150073/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:1153372/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:3044417/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:2594235/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:1851611/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:1788530/1‑71 (MQ=255)
      ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg                           >  1:1250619/1‑71 (MQ=255)
                            aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc     <  1:1210811/71‑1 (MQ=255)
                             aGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc     <  1:4010710/70‑1 (MQ=255)
                               cGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAag  <  1:393375/71‑1 (MQ=255)
                                                 |                                                    
TGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAG  >  W3110S.gb/1032442‑1032543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: