| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,032,491 | 0 | C | T | 50.0% | ‑5.3 / 30.2 | 22 | intergenic (‑357/‑70) | serT/hyaA | tRNA‑Ser/hydrogenase 1, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (11/0); new base T (6/5); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAG > W3110S.gb/1032442‑1032543 | tGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt < 1:2928354/63‑1 (MQ=255)tGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt < 1:3735165/63‑1 (MQ=255) aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATATGGACGTCGCTTGCCGACCAt > 1:366319/1‑70 (MQ=255) aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt > 1:1740431/1‑70 (MQ=255) aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt > 1:4038596/1‑70 (MQ=255) aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt > 1:3985053/1‑70 (MQ=255) aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt > 1:217984/1‑70 (MQ=255) aGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCAt > 1:270171/1‑70 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:313322/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:606869/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:4082860/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:3982703/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:3150073/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:1153372/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:3044417/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:2594235/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:1851611/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:1788530/1‑71 (MQ=255) ttCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAgcg > 1:1250619/1‑71 (MQ=255) aaGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:1210811/71‑1 (MQ=255) aGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGc < 1:4010710/70‑1 (MQ=255) cGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAag < 1:393375/71‑1 (MQ=255) | TGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAG > W3110S.gb/1032442‑1032543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |