| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,426,674 | 0 | T | C | 72.4% | 93.3 / 41.1 | 58 | D393D (GAT→GAC) | trkG | potassium transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/16); new base C (15/27); total (15/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.58e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTA > W3110S.gb/1426609‑1426735 | aTAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:2605806/71‑1 (MQ=255)aTAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:1279014/71‑1 (MQ=255)aTAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:2520064/71‑1 (MQ=255) tAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:1826529/70‑1 (MQ=255) tCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATTGTTACAGACCgtgt < 1:2953661/66‑1 (MQ=255) tCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:1843720/66‑1 (MQ=255) tctcATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTg < 1:25617/70‑1 (MQ=255) tctcATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTg < 1:1742582/70‑1 (MQ=255) tctcATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTg < 1:1984547/70‑1 (MQ=255) agCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:1637059/48‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:86385/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:769656/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:795627/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:2181878/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:3019370/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:3033391/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:3175607/71‑1 (MQ=255) gCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:3395262/71‑1 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:307037/1‑71 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:1267524/1‑71 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:3700994/1‑71 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:3985214/1‑71 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:258515/1‑71 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:2196670/1‑71 (MQ=255) gttgttGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:2039064/1‑71 (MQ=255) aGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:727401/71‑1 (MQ=255) aGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:671454/71‑1 (MQ=255) aGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:3768883/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTtata < 1:1229433/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTtata < 1:4076120/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTtata < 1:4009763/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTtata < 1:3336915/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTtata < 1:2731004/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTtata < 1:3062510/71‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTgatc > 1:2104109/1‑70 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTgatc > 1:825719/1‑70 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTgatc > 1:2903592/1‑70 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTgatc > 1:444801/1‑70 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTgatc > 1:1814236/1‑70 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTgatc > 1:1635458/1‑70 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTATTCTTgatc > 1:3431345/1‑70 (MQ=255) gtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:2787728/59‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAc < 1:2034428/70‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAc < 1:3502673/70‑1 (MQ=255) aaaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAc < 1:611682/69‑1 (MQ=255) ggAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttctt < 1:1812448/46‑1 (MQ=255) aTAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:3451986/71‑1 (MQ=255) aTAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:601629/71‑1 (MQ=255) aTAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:743156/71‑1 (MQ=255) aTAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:1590553/71‑1 (MQ=255) aGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta > 1:3467264/1‑71 (MQ=255) ttACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:469454/69‑1 (MQ=255) ttACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:1607119/69‑1 (MQ=255) ttACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:794334/69‑1 (MQ=255) ttACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:1584075/69‑1 (MQ=255) ttACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:3821906/69‑1 (MQ=255) ttACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:3791860/69‑1 (MQ=255) ttACAGATCGTGTAAAGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:3440438/69‑1 (MQ=255) | ATAAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTA > W3110S.gb/1426609‑1426735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |