Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,204,480	0	T	C	66.7%	17.1 / 12.4	18	Y290H (TAT→CAT)	yehI	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base C (0/12);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 5.39e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAC  >  W3110S.gb/2204441‑2204544                                        |                                                                 cgcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGCATGCCCATAAAGAAGATTGCGGCAGTctgct                                   <  1:1774919/71‑1 (MQ=255) cgcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGCATGCCCATAAAGAAGATTGCGGCAGTctgct                                   <  1:3854164/71‑1 (MQ=255)                        tCCGCTCCTCGCGATTCATGCCCATAAAGAAGATTGCGGCAGTctgct                                   <  1:3879617/48‑1 (MQ=255)                         ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGt           >  1:797455/1‑71 (MQ=255)                         ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGt           >  1:339299/1‑71 (MQ=255)                         ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGt           >  1:3353544/1‑71 (MQ=255)                         ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGt           >  1:2888099/1‑71 (MQ=255)                         ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGt           >  1:2411351/1‑71 (MQ=255)                         ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGt           >  1:1881327/1‑71 (MQ=255)                          cgcgCCTCGCGATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTc          <  1:362499/71‑1 (MQ=255)                          cgcgCCTCGCGATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTc          <  1:3752801/71‑1 (MQ=255)                          cgcgCCTCGCGATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTc          <  1:3796581/71‑1 (MQ=255)                              ccTCGCGATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATc       <  1:3440845/70‑1 (MQ=255)                                  gcgATGCATTCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAc  <  1:1199948/71‑1 (MQ=255)                                  gcgATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAc  <  1:3179258/71‑1 (MQ=255)                                  gcgATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAc  <  1:3532780/71‑1 (MQ=255)                                  gcgATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAc  <  1:3559597/71‑1 (MQ=255)                                  gcgATGCATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAc  <  1:3644741/71‑1 (MQ=255)                                        |                                                                 CGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCAC  >  W3110S.gb/2204441‑2204544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑