| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,239,880 | 0 | T | G | 50.0% | 3.3 / 13.7 | 14 | intergenic (+46/+197) | yeiA/mglC | predicted oxidoreductase/methyl‑galactoside transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (0/7); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGAT > W3110S.gb/2239812‑2239944 | aagaaCACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTg < 1:3881141/71‑1 (MQ=255) aaaCCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGc > 1:1589885/1‑54 (MQ=255) aaaCCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACaa > 1:1795360/1‑70 (MQ=255) ccTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTa < 1:2105657/70‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:1408477/71‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:1739072/71‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:2340806/71‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:3925186/71‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:402988/71‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:489063/71‑1 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGc < 1:2740932/70‑1 (MQ=255) gCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGAt > 1:2557122/1‑70 (MQ=255) gCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGAt > 1:3019377/1‑70 (MQ=255) ggCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTa < 1:3569677/55‑1 (MQ=255) | AAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGAT > W3110S.gb/2239812‑2239944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |