| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,504,884 | 0 | T | C | 52.2% | ‑2.1 / 29.9 | 23 | V256V (GTT→GTC) | ypdA | predicted sensory kinase in two‑component system with YpdB |
| Reads supporting (aligned to +/- strand): ref base T (10/1); new base C (12/0); total (22/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAT > W3110S.gb/2504830‑2504953 | cATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACAt < 1:3713890/70‑1 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGc > 1:2832630/1‑54 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTa > 1:966595/1‑71 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTa > 1:852307/1‑71 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTa > 1:678431/1‑71 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTa > 1:394148/1‑71 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTa > 1:2993756/1‑71 (MQ=255) cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTa > 1:256457/1‑71 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:2853959/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:501700/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:4128133/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:4000769/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:3066384/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:294036/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:2838615/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:2486323/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:1346306/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:2019719/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:1565415/3‑58 (MQ=255) ctgATCATGTGCTGGCCTATGTCGGCGTGGGTGAACACAACTATCAGAATGGCGATGa > 1:1742914/3‑58 (MQ=255) ccGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGaa > 1:741946/1‑49 (MQ=255) ccGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGaa > 1:695640/1‑49 (MQ=255) ccGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGaa > 1:1698569/1‑49 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCTATGAAt < 1:532350/71‑1 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa > 1:3953672/1‑70 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa > 1:1728576/1‑70 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa > 1:2571157/1‑70 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa > 1:2222236/1‑70 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa > 1:1013343/1‑70 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt < 1:3749865/71‑1 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt < 1:1442780/71‑1 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt < 1:2278709/71‑1 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt < 1:2435722/71‑1 (MQ=255) ttGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt < 1:1175732/71‑1 (MQ=255) | CATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAT > W3110S.gb/2504830‑2504953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |