| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,669,838 | 0 | T | C | 73.9% | 78.9 / 28.6 | 46 | V92A (GTT→GCT) | hcaC | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/12); new base C (22/12); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.61e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTC > W3110S.gb/2669772‑2669903 | gCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGa < 1:1908152/70‑1 (MQ=255) ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa < 1:3796274/70‑1 (MQ=255) ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa < 1:2259952/70‑1 (MQ=255) ccTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa < 1:2799659/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACTACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:3817623/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:534984/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:2442821/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:1755922/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:3401237/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:3360360/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:2607573/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:3088771/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:2203897/70‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:2193427/70‑1 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:2270505/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:3009812/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:2930790/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:3327903/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:3455288/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:4004401/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:876287/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:2265758/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:1363878/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:20818/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:1823328/1‑38 (MQ=255) cccGCCACCGATCCGCTCACCACTTATCCAGTACACGt > 1:1983743/1‑38 (MQ=255) tcTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:2366959/42‑1 (MQ=255) ccAGTACACGTTGAAGGTGGTGACATTTTCATCGACt < 1:3640868/37‑1 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAAc > 1:3854276/1‑70 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAAc > 1:3650331/1‑70 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACAGGAGGCGCAGCCATGAGCGATCTGCATaa > 1:214806/1‑69 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:1231258/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:863905/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:820761/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:3712314/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:1835710/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:2057878/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:2612117/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc < 1:1054863/60‑1 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAg > 1:4091665/1‑71 (MQ=255) gTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAg > 1:3072119/1‑71 (MQ=255) tACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGt > 1:2262904/1‑71 (MQ=255) tACACGCTGAAGGTGGGGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGt > 1:1359913/1‑71 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGt > 1:3142816/1‑69 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:2067188/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:3806997/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:1789428/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:3639325/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:3874976/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:2749548/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:3631622/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:487866/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:60542/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:323010/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:2223342/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:1984016/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAAAGAGTc > 1:3703675/1‑70 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACAGGAGGCGCAGCCATGAGCGATCTGCATAACGAg > 1:3037496/1‑68 (MQ=255) | GCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTC > W3110S.gb/2669772‑2669903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |